{"id":22510,"date":"2022-09-28T12:01:24","date_gmt":"2022-09-28T12:01:24","guid":{"rendered":"https:\/\/todaysveterinarybusiness.com\/?p=22510"},"modified":"2022-10-27T13:28:53","modified_gmt":"2022-10-27T13:28:53","slug":"nextmune-pet-allergy-xplorer","status":"publish","type":"post","link":"https:\/\/navc.sitepreview.app\/todaysveterinarybusiness.com\/nextmune-pet-allergy-xplorer\/","title":{"rendered":"Nextmune Pet Allergy Xplorer"},"content":{"rendered":"<p>This article is part of the <a href=\"https:\/\/todaysveterinarybusiness.com\/column\/behind-the-design\/\" target=\"_blank\" rel=\"noopener\">Behind the Design series<\/a> published in the <a href=\"https:\/\/todaysveterinarybusiness.com\/category\/clinic-innovation-guide-2022\/\" target=\"_blank\" rel=\"noopener\">2022 Clinic Innovation Guide<\/a>. This article explores the design process for Nextmune&#8217;s Pet Allergy Xplorer, from the perspective from a key leader in the process.<\/p>\n<hr \/>\n<h2 class=\"p1\"><span class=\"s1\">The Problem<\/span><\/h2>\n<p class=\"p2\"><span class=\"s1\">Traditionally, allergy testing in veterinary medicine involves placing an allergen extract on an enzyme-linked immunoassay <\/span>(ELISA) plate to incubate the serum<span class=\"s1\"> and then administering a reagent that recognizes immunoglobulin E (IgE). The resultant color reaction indicates how much IgE is present. <\/span><\/p>\n<p class=\"p3\"><span class=\"s1\">This technique, which is used by veterinary laboratories worldwide, has not changed for decades. However, results can vary considerably, depending on the extract used, and false negatives can occur if clinically relevant protein allergen concentrations are not sufficient. For example, the extract for the house dust mite, a common pet allergen, is made by grinding the mite, adding solvents to release the allergenic proteins and<br \/>\npurifying the proteins. <\/span><\/p>\n<p class=\"p3\"><span class=\"s1\">The house dust mite contains more than 10,000 proteins, but only about 40 that cause an allergic reaction are recognized. This means a low percentage of the allergy- causing proteins are seen when an extract is evaluated, especially if a pet has a low IgE level against a particular allergen. This can easily result in a false negative. In addition, extracts can vary not just between laboratories but also allergenic extract, making results hard to reproduce.<\/span><\/p>\n<div class=\"su-quote su-quote-style-default\"><div class=\"su-quote-inner su-u-clearfix su-u-trim\">THE DIFFERENCE MAKER<\/p>\n<p>Pet Allergy Xplorer (PAX) is the first commercial serological IgE-specific test that uses allergen extracts and molecular components to identify which allergens are affecting pets.<\/div><\/div>\n<h2 class=\"p1\"><span class=\"s1\">The Solution<\/span><\/h2>\n<p class=\"p2\"><span class=\"s1\">To gain more accurate and sensitive information, tests are needed to identify each individual allergenic protein. Instead of testing for the house dust mite (or any particular allergen) as a whole, techniques are needed to test for the specific proteins that cause an allergic reaction. Human practitioners use molecular allergology to determine the allergens causing problems to provide their patients with a better level of care.<\/span><\/p>\n<p class=\"p3\"><span class=\"s1\">MacroArray Diagnostics launched the Allergy Explorer (ALEX), which provides a sensitization profile for human patients based on a test panel composed of allergen extracts and molecular allergens. Since its founding in 2016, the company has launched two generations of ALEX, offering a panel that covers nearly 100% of the world\u2019s relevant allergens. It also developed the Food Xplorer (FOX), to detect IgG-mediated food intolerances.<\/span><\/p>\n<h2 class=\"p1\"><span class=\"s1\">The Innovation<\/span><\/h2>\n<p class=\"p2\"><span class=\"s1\">After experiencing allergies myself, and being tested using molecular allergology, I thought the technology could be extremely beneficial to veterinary medicine. <\/span><\/p>\n<p class=\"p3\"><span class=\"s1\">This led to Nextmune partnering with Macro Array Diagnostics to develop the Pet Allergy Xplorer (PAX), the first commercial serological IgE-specific test that uses allergen extracts and molecular components to identify which allergens are affecting pets. Advantages include:<\/span><\/p>\n<ul>\n<li class=\"p4\"><b>Improved reproducibility <\/b>\u2014 A state-of-the-art robot builds each ELISA testing array, providing a uniform production method that exceeds current ELISA plate-building reproducibility. In addition, a standardization process is used to make the allergenic extracts, improving reproducibility.<\/li>\n<li class=\"p4\"><b>Increased data <\/b>\u2014 Typical allergy testing via serum provides about 90 results, and intradermal testing provides around 60 to 80 results. The PAX cartridge holds 300 positions \u2014 100 of which will be extracts and 200 will be molecular components.<\/li>\n<li class=\"p4\"><b>Automated technology <\/b>\u2014 Robots are used throughout the process, decreasing human error. The microarray dots during the plate manufacturing process are dispensed by a robot. In addition, a robot pipettes the serum during the testing phase. Controls are built into the PAX cartridge to ensure the sample has sufficient IgE for testing, and these controls also indicate if the cartridge is used properly.<\/li>\n<li class=\"p4\"><b>Improved treatment <\/b>\u2014 The more accurate and sensitive results that PAX provides will improve the ability to produce an effective hyposensitization therapy that <span class=\"s2\">will provide more relief for the pet.<\/span><\/li>\n<li class=\"p4\"><b>Improved cross-reactivity identification <\/b>\u2014 Using allergen extracts and molecular components in one test will help identify allergenic cross-reactivity.<\/li>\n<li class=\"p4\"><b>Elucidated polysensitization <\/b>\u2014 When multiple allergens cause a reaction, PAX will help identify the primary offenders.<\/li>\n<li class=\"p4\"><b>Individualized results <\/b>\u2014 The results provided to the veterinarian will be specific to the region where they live, as well as the pet\u2019s species.<\/li>\n<li class=\"p4\"><b>Improved accuracy <\/b>\u2014 PAX uses <span class=\"s2\">a single well-characterized anti-IgE<\/span> monoclonal antibody to detect pet IgE, ensuring a detection level. PAX also uses technology to block cross-reactive carbohydrate determinants (CCD). In some cases, CCDs bind to IgE receptors, creating false positives. The PAX technology uses a blocking agent to prevent this binding. Allergy tests that do not use CCD blockers identify numerous <span class=\"s2\">allergens, many of which are irrelevant.<\/span> This technology ensures only the pertinent allergens are identified to prevent confusion.<\/li>\n<li class=\"p5\"><b>Ongoing development <\/b>\u2014 The cartridges currently contain the allergenic components and extracts that prevailing research indicates are clinically relevant. As data is gathered through testing, other allergenic components may be discovered. For example, if an extract continues to indicate positive while the individual molecular components indicate negative, we need to determine if another unidentified component is present or a cross reactivity is occurring. This data will help us characterize molecules to add in future versions, revolutionizing the research in pet allergies.<\/li>\n<\/ul>\n<p class=\"p2\"><span class=\"s1\">Nextmune is the only veterinary diagnostic laboratory currently using molecular allergology. This next-generation test will allow veterinarians to more accurately and sensitively diagnose allergic pets to facilitate treatment strategies. The initial launch for dogs is scheduled for January 2023, <\/span>followed by tests for cats and horses.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Nextmune is the only veterinary diagnostic laboratory currently using molecular allergology.<\/p>\n","protected":false},"author":236,"featured_media":22512,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"iawp_total_views":0,"footnotes":""},"categories":[392],"tags":[],"class_list":["post-22510","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-clinic-innovation-guide-2022","column-behind-the-design","clinical_topics-innovation-technology"],"acf":{"hide_sidebar":false,"hide_sidebar_ad":false,"hide_all_ads":false},"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v24.7 (Yoast SEO v27.3) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>Nextmune Pet Allergy Xplorer 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